All I can say is that if 'HIV' testing was a mess in the past, it's now utter chaos. All the problems the PG have documented with the Western Blot and ELIZA are all still there, and now we have more criteria thrown into the mix. And yes, using one antibody test compared to another one from a different manufacturer is absolutely hilarious. All of this screams of a medical system doing everything it can to keep 'HIV disease' alive and well via obfuscation.
I was literally laughing out loud when I read that. I thought it was so funny I called my husband on his lunch break so I’d have someone to laugh with. But seriously — how can these tests be so bad that even the same test manufactured by a different company is somehow considered “orthogonal” to an identical test. What the HELL are these tests detecting? That’s the question we need to focus on.
Most scientific experiments involve mixing invisible components dissolved in indistinguishable clear liquids and using some complicated mechanical machine/proprietary software combination to measure a signal (typically light or color) which must then be interpreted as to its significance. Having run hundreds of ELISA's and PCR reactions over 30yrs, few methodologies are as fraught with potential for improper procedure problems and misinterpretation of data as they are.
Sample preparation is critical- how much measurable starting material is there, and what is the biological condition of the material (are proteins denatured, is DNA degraded?) ELISAs require a pair of antibody solutions which vary widely from one manufacturer to another and are prone to degradation. Antibodies must both be diluted- what is the proper dilution- this must be correctly determined. Incubation times are critical, which blocking solution to use, how many between addition wash steps and for how long, which detection method to use (luminescent, colorimetric, IR,) which detection machine is used, and ELISA plates have notoriously uneven protein binding characteristics (one review paper found only 2 of 50 plates tested that bound protein well.)
PCR reactions must have properly designed primers, buffer conditions must be optimized for each primer pair (for instance, 1mM, 2mM or 4mM MgCl2,) what DNA polymerase is used, what is the biological condition of the polymerase protein, what cycling parameters are used, what is the minimum and maximum number of cycles that yield statistically significant results, what background controls are used, a single housekeeping gene such as actin or a set of different genes, as single genes are no longer considered adequate?
Lastly, what positive control does one use in a PCR reaction designed to amplify a supposed sequence from a viral genome? The only true control would be a purified virus solution, but as that procedure has never been described in any scientific journal, then what experiment are we talking about runnin? Even in the unlikely case that one could find a technologist running these "experiments" who could explain exactly what happens in their kit mixture-derived reactions, following the scientific method without a true positive control is impossible. So these tests are all bunk even if all the many variables were controlled for (which is highly unlikely.)
About 10yrs into my career I discovered that nobody understands what goes on in the incredibly complex biological system known as life, and everybody implicitly agrees not to reveal that fact, and instead, pretend we understand so as to keep grant funding coming and research publications going. Even now, because I have many friends still in the game, I don't reveal everything. Science and Medicine are one big speculation game with fancy pictures of models and biochemical pathways used to fool the layman- but it's one giant con game.
David, can you email me? I’d love to ask you some questions about your experience working on this if you’re comfortable with that. You can reply to the post or email me at walkerpercyfan at gmail. Thanks!
All I can say is that if 'HIV' testing was a mess in the past, it's now utter chaos. All the problems the PG have documented with the Western Blot and ELIZA are all still there, and now we have more criteria thrown into the mix. And yes, using one antibody test compared to another one from a different manufacturer is absolutely hilarious. All of this screams of a medical system doing everything it can to keep 'HIV disease' alive and well via obfuscation.
I was literally laughing out loud when I read that. I thought it was so funny I called my husband on his lunch break so I’d have someone to laugh with. But seriously — how can these tests be so bad that even the same test manufactured by a different company is somehow considered “orthogonal” to an identical test. What the HELL are these tests detecting? That’s the question we need to focus on.
"HIV disease" is boggling my mind. I guess it saves the awkwardness of saying "may lead to AIDS"
They’ve been calling it “HIV disease” for years to mask the lack of evidence of causation.
Thank you, Miss Rebecca.
Most scientific experiments involve mixing invisible components dissolved in indistinguishable clear liquids and using some complicated mechanical machine/proprietary software combination to measure a signal (typically light or color) which must then be interpreted as to its significance. Having run hundreds of ELISA's and PCR reactions over 30yrs, few methodologies are as fraught with potential for improper procedure problems and misinterpretation of data as they are.
Sample preparation is critical- how much measurable starting material is there, and what is the biological condition of the material (are proteins denatured, is DNA degraded?) ELISAs require a pair of antibody solutions which vary widely from one manufacturer to another and are prone to degradation. Antibodies must both be diluted- what is the proper dilution- this must be correctly determined. Incubation times are critical, which blocking solution to use, how many between addition wash steps and for how long, which detection method to use (luminescent, colorimetric, IR,) which detection machine is used, and ELISA plates have notoriously uneven protein binding characteristics (one review paper found only 2 of 50 plates tested that bound protein well.)
PCR reactions must have properly designed primers, buffer conditions must be optimized for each primer pair (for instance, 1mM, 2mM or 4mM MgCl2,) what DNA polymerase is used, what is the biological condition of the polymerase protein, what cycling parameters are used, what is the minimum and maximum number of cycles that yield statistically significant results, what background controls are used, a single housekeeping gene such as actin or a set of different genes, as single genes are no longer considered adequate?
Lastly, what positive control does one use in a PCR reaction designed to amplify a supposed sequence from a viral genome? The only true control would be a purified virus solution, but as that procedure has never been described in any scientific journal, then what experiment are we talking about runnin? Even in the unlikely case that one could find a technologist running these "experiments" who could explain exactly what happens in their kit mixture-derived reactions, following the scientific method without a true positive control is impossible. So these tests are all bunk even if all the many variables were controlled for (which is highly unlikely.)
About 10yrs into my career I discovered that nobody understands what goes on in the incredibly complex biological system known as life, and everybody implicitly agrees not to reveal that fact, and instead, pretend we understand so as to keep grant funding coming and research publications going. Even now, because I have many friends still in the game, I don't reveal everything. Science and Medicine are one big speculation game with fancy pictures of models and biochemical pathways used to fool the layman- but it's one giant con game.
David, can you email me? I’d love to ask you some questions about your experience working on this if you’re comfortable with that. You can reply to the post or email me at walkerpercyfan at gmail. Thanks!